Dr Anestis Tsakiridis

Anestis Tsakiridis

Vice-Chancellor’s Fellow
Lecturer

Department of Biomedical Science
The University of Sheffield
E225, Alfred Denny building
Western Bank
Sheffield S10 2TN
United Kingdom

Tel: +44 114 222 2367
Email: a.tsakiridis@sheffield.ac.uk
Website: Tsakiridis Lab

Centre for Stem Cell Biology  Bateson Centre
Cell Biology and Cancer  Developmental Biology
Stem Cells and Regenerative Medicine  Neuroscience

General

Brief career history

  • 2016-present: Group Leader (Vice-Chancellor’s Fellow)
  • 2009-2015: Post-doctoral researcher, MRC Centre for Regenerative Medicine, University of Edinburgh (Advisor: Prof. Val Wilson)
  • 2006-2009: Post-doctoral researcher, Institute for Stem Cell Research, University of Edinburgh (Advisor: Prof. Josh Brickman)
  • 2002-2006: PhD, University of Edinburgh (Advisors: Prof. Lesley Forrester, Prof. Josh Brickman)
  • 2001-2002: MSc (Res), University of Edinburgh
  • 1998-2001: BSc (Hons), University of Edinburgh

Research interests

We aim to define the molecular basis of cell fate decisions during human embryonic development through the use of human pluripotent stem cells (hPSCs) and exploit this knowledge for the efficient in vitro  generation of clinically relevant human cell populations.




Professional activities

  • Member of the British Society for Developmental Biology (BSDB), International Society for Stem Cell Research (ISSCR), British Society for Gene and Cell Therapy (BSGCT), Anatomical Society.Full publications
Research

We aim to define the molecular basis of cell fate decisions during human embryonic development through the use of human pluripotent stem cells (hPSCs). We focus particularly on the in vitro modelling of the events guiding the “birth” of the spinal cord and trunk musculature from a common precursor population known as Neuromesodermal Progenitors (NMPs) during embryonic axis elongation.

We are also interested in another multipotent embryonic progenitor population known as the neural crest (NC). Depending on their position along the anteroposterior (A-P) axis, NC cells give rise to various specialised cell types, such as peripheral neurons, neuroendocrine cells and skeletal cells in the head. We have defined the culture conditions directing  the generation of  NMPs from hPSCs as well as their differentiation into either neural or mesodermal cells (Gouti, Tsakiridis et al., 2014 PloS Biology; Frith et al. 2018 eLife). We have also established efficient protocols for the in vitro production of human NC cells corresponding to most A-P levels and found a close link between NMPs and the precursors of trunk NC cells (Frith et al. 2018 eLife).

Using these in vitro systems we are trying to address the following questions:

1) How do embryonic cells proceed from pluripotency to NM bipotency?
2) What are the molecular determinants of NM bipotency and differentiation?
3) How is axial identity shaped and maintained in human embryonic cells?
4) How do “altered” embryonic multipotent states drive tumourigenesis

Our ultimate goal is to exploit the knowledge from addressing the above questions in order to achieve the precise and efficient engineering of NMP- and NC-derived cell types for regenerative medicine and disease-modelling applications.

Funding:

  • Biotechnology and Biological Sciences Research Council (BBSRC)
  • The Royal Society
  • Children's Cancer and Leukaemia Group/Little Princess Trust
  • MRC Discovery Me
  • dicine North Doctoral Training Partnership
  • EU Horizon2020
  • Neuroblastoma UK
Teaching

Undergraduate and postgraduate taught modules

Undergraduate:

  • BMS110  Research Topics in Biomedicine
  • BMS247 Biology of Stem Cells, Ageing and Cancer (Co-ordinator)
  • BMS336 Modelling Human Disease
  • Level 3 Practical and Dissertation Modules

Masters (MSc):

  • BMS6336  Modelling Human Disease and Dysfunction
Opportunities

We welcome speculative applications. Please email your CV and research interests to:

Email: a.tsakiridis@sheffield.ac.uk

Selected publications

Journal articles