Dr Anestis Tsakiridis

All publications

Journal articles

• Wong FCK, Zhang M, Thomson E, Schumacher LJ, Tsakiridis A, Ashmore J, Li T, Blin G, Karagianni E, Mullin NP , Chambers I et al (2025) NANOG is repurposed after implantation to repress Sox2 and begin pluripotency extinction. The EMBO Journal, 44(19), 5337-5374. View this article in WRRO
• Fang W, Cheng B, Pastor SJ, Barbaric I, Tsakiridis A, Porras T, Wu H-W, Parekh C, Asgharzadeh S, Maris JM & Huang M (2025) Abstract 2600: A human stem cell model of neuroblastoma reveals candidate drivers of tumorigenesis on chromosome 17q. Cancer Research, 85(8_Supplement_1), 2600-2600.
• Jevans B, Cooper F, Fatieieva Y, Gogolou A, Kang Y-N, Restuadi R, Moulding D, Vanden Berghe P, Adameyko I, Thapar N , Andrews PW et al (2024) Human enteric nervous system progenitor transplantation improves functional responses in Hirschsprung disease patient-derived tissue. Gut, 73(9), 1441-1453. View this article in WRRO
• Saldana-Guerrero IM, Montano-Gutierrez LF, Boswell K, Hafemeister C, Poon E, Shaw LE, Stavish D, Lea RA, Wernig-Zorc S, Bozsaky E , Fetahu IS et al (2024) A human neural crest model reveals the developmental impact of neuroblastoma-associated chromosomal aberrations. Nature Communications, 15(1). View this article in WRRO
• Cooper F, Souilhol C, Haston S, Gray S, Boswell K, Gogolou A, Frith TJR, Stavish D, James BM, Bose D , Dale JK et al (2024) Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors. Development, 151(3). View this article in WRRO
• (2024) The people behind the papers – Fay Cooper and Anestis Tsakiridis. Development, 151(3).
• Saldana-Guerrero IM, Montano-Gutierrez LF, Hafemeister C, Stavish D, Shaw LE, Fetahu IS, Wenninger-Weinzierl A, Sturtzel C, Souilhol C, Tarelli S , Shoeb MR et al (2023) Abstract 3542: A stem cell model dissects detrimental effects of neuroblastoma-linked chromosomal aberrations on cell differentiation during neural crest development. Cancer Research, 83(7_Supplement), 3542-3542.
• Gogolou A, Souilhol C, Granata I, Wymeersch FJ, Manipur I, Wind M, Frith TJR, Guarini M, Bertero A, Bock C , Halbritter F et al (2022) Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor. eLife, 11. View this article in WRRO
• Cooper F & Tsakiridis A (2022) Towards clinical applications of in vitro-derived axial progenitors. Developmental Biology, 489, 110-117. View this article in WRRO
• Cooper F & Tsakiridis A (2022) Shaping axial identity during human pluripotent stem cell differentiation to neural crest cells. Biochemical Society Transactions, 50(1), 499-511. View this article in WRRO
• Wind M & Tsakiridis A (2021) In vitro generation of posterior motor neurons from human pluripotent stem cells. Current Protocols, 1(9).
• Gogolou A, Frith TJR & Tsakiridis A (2021) Generating enteric nervous system progenitors from human pluripotent stem cells. Current Protocols, 1(6).
• Wind M, Gogolou A, Manipur I, Granata I, Butler L, Andrews PW, Barbaric I, Ning K, Guarracino MR, Placzek M & Tsakiridis A (2021) Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives. Development, 148(6). View this article in WRRO
• Wymeersch FJ, Wilson V & Tsakiridis A (2021) Understanding axial progenitor biology in vivo and in vitro. Development, 148(4). View this article in WRRO
• Frith TJR, Gogolou A, Hackland JOS, Hewitt ZA, Moore HD, Barbaric I, Thapar N, Burns AJ, Andrews PW, Tsakiridis A & McCann CJ (2020) Retinoic acid accelerates the specification of enteric neural progenitors from in-vitro-derived neural crest. Stem Cell Reports, 15(3), 557-565. View this article in WRRO
• Ruske LJ, Kursawe J, Tsakiridis A, Wilson V, Fletcher AG, Blythe RA & Schumacher LJ (2020) Coupled differentiation and division of embryonic stem cells inferred from clonal snapshots. Physical Biology, 17(6). View this article in WRRO
• Frith TJR & Tsakiridis A (2019) Efficient generation of trunk neural crest and sympathetic neurons from human pluripotent stem cells via a neuromesodermal axial progenitor intermediate. Current Protocols in Stem Cell Biology. View this article in WRRO
• Sutherland L, Ruhe M, Gattegno-Ho D, Mann K, Greaves J, Koscielniak M, Meek S, Lu Z, Waterfall M, Taylor R , Tsakiridis A et al (2018) LIF-dependent survival of embryonic stem cells is regulated by a novel palmitoylated Gab1 signalling protein. Journal of Cell Science, 131(18). View this article in WRRO
• Lopez-Yrigoyen M, Fidanza A, Cassetta L, Axton RA, Taylor AH, Meseguer-Ripolles J, Tsakiridis A, Wilson V, Hay DC, Pollard JW & Forrester LM (2018) A human iPSC line capable of differentiating into functional macrophages expressing ZsGreen: a tool for the study and in vivo tracking of therapeutic cells. Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 373(1750). View this article in WRRO
• Economou C, Tsakiridis A, Wymeersch FJ, Gordon-Keylock S, Dewhurst RE, Fisher D, Medvinsky A, Smith AJH & Wilson V (2015) Intrinsic factors and the embryonic environment influence the formation of extragonadal teratomas during gestation. BMC Developmental Biology, 15(1).
• Tsakiridis A & Wilson V (2015) Assessing the bipotency of in vitro-derived neuromesodermal progenitors.. F1000Research, 4. View this article in WRRO
• Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2015) Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development, 142(4), 809-809.
• Gouti M, Tsakiridis A, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V & Briscoe J (2014) In Vitro Generation of Neuromesodermal Progenitors Reveals Distinct Roles for Wnt Signalling in the Specification of Spinal Cord and Paraxial Mesoderm Identity. PLoS Biology, 12(8). View this article in WRRO
• Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S & Wilson V (2014) Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors. Development, 141(6), 1209-1221. View this article in WRRO
• Huang Y, Osorno R, Tsakiridis A & Wilson V (2012) In Vivo Differentiation Potential of Epiblast Stem Cells Revealed by Chimeric Embryo Formation. Cell Reports, 2(6), 1571-1578.
• Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I & Wilson V (2012) The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression. Journal of Cell Science, 125(12), e1-e1.
• Osorno R, Tsakiridis A, Wong F, Cambray N, Economou C, Wilkie R, Blin G, Scotting PJ, Chambers I & Wilson V (2012) The developmental dismantling of pluripotency is reversed by ectopic Oct4 expression. Development, 139(13), 2288-2298.
• Tan CL, Tsakiridis A, Noor DAM, Walker DA, Wilson V & Scotting PJ (2012) REASSESSING THE ORIGINS OF INTRACRANIAL GERM CELL TUMOUR FORMATION. PEDIATRIC BLOOD & CANCER, 59(6), 1089-1089.
• Brickman JM, Tsakiridis A, To C & Stanford WL (2010) A Wider Context for Gene Trap Mutagenesis, 271-295.
• Tsakiridis A, Tzouanacou E, Rahman A, Colby D, Axton R, Chambers I, Wilson V, Forrester L & Brickman JM (2009) Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells. Nucleic Acids Research, 37(19). View this article in WRRO
• Tsakiridis A, Alexander LM, Gennet N, Sanchez-Martin RM, Livigni A, Li M, Bradley M & Brickman JM (2009) Microsphere-based tracing and molecular delivery in embryonic stem cells. Biomaterials, 30(29), 5853-5861.
• Sanchez‐Martin RM, Alexander L, Muzerelle M, Cardenas‐Maestre JM, Tsakiridis A, Brickman JM & Bradley M (2009) Microsphere‐Mediated Protein Delivery into Cells. ChemBioChem, 10(9), 1453-1456.
• Tsakiridis A, Tzouanacou E, Larralde O, Watts TM, Wilson V, Forrester L & Brickman JM (2007) A novel triple fusion reporter system for use in gene trap mutagenesis. genesis, 45(6), 353-360.
• Sabahi-Kaviani R, Gogolou A, Souilhol C, van der Kroeg M, Kushner SA, de Vrij FMS, Tsakiridis A & Luttge R () Nervous System-on-Chip: Innovative Microfluidic Platform to Compartmentalize hiPSC-Derived Neural Networks. Micromachines, 17(2), 199-199.
• Harron KL, Jevans BS, Allin BSR, Davidson JR, Tsakiridis A, Andrews PW, De Coppi P & McCann CJ () Childhood outcomes in children with Hirschsprung disease: a population-based data linkage study in England. Frontline Gastroenterology.
• Frith TJR, Granata I, Wind M, Stout E, Thompson O, Neumann K, Stavish D, Heath PR, Ortmann D, Hackland JOS , Anastassiadis K et al () Human axial progenitors generate trunk neural crest cells in vitro. eLife, 7.
• Tsakiridis A & Wilson V () Assessing the bipotency of in vitro-derived neuromesodermal progenitors. F1000Research, 4, 100-100.

Conference proceedings

• Jevans B, Cooper F, Peruzza L, Perin S, Li S, Mcateer T, Tsakiridis A & Mccann C (2025) In-depth characterization of the colonic pathology of the B6;129-Ednrbtm1Ywa/J mouse model of Hirschsprung disease and stem cell-based rescue. NEUROGASTROENTEROLOGY AND MOTILITY, Vol. 37
• Kaviani RS, Shiryaeva M, Souilhol C, Wind M, Tsakiridis A & Luttge R (2023) Enhancing neuronal cell network organization and activity using NOA81 and PDMS nanogrooves. TISSUE ENGINEERING PART A, Vol. 29(13-14)
• Frith TJR, McCann C, Gogolou A, Thapar N, Tsakiridis A, Burns A & Andrews PW (2019) Transplantation of hPSC derived enteric neural progenitors as a basis to derive a preclinical cell therapy for Hirschsprung's disease. Human Gene Therapy, Vol. 30(8) (pp P02-P02). Sheffield, UK, 19 June 2019 - 19 June 2019. View this article in WRRO
• Wymeersch F, Skylaki S, Huang Y, Tsakiridis A, Economou C, Marek-Johnson C, Tomlinson S & Wilson V (2017) Primitive Streak and Tail Bud Progenitor Populations drive Patterning of the Anteroposterior and Mediolateral Axes. Mechanisms of Development, Vol. 145 (pp S82-S82)
• Tsakiridis A, Tzouanacou E, Rahman A, Axton R, Wilson V, Forrester L & Brickman J (2009) 17-P033 Gene trap vectors for unbiased and expression-independent mutagenesis in embryonic stem cells. Mechanisms of Development, Vol. 126 (pp S280-S280)

Preprints

• Harron KL, Jevans B, Allin BSR, Davidson JR, Tsakiridis A, Andrews PW, De Coppi P & McCann CJ (2025) Childhood outcomes in children with Hirschsprung disease: a population-based data linkage study in England, Cold Spring Harbor Laboratory.
• Jevans B, Cooper F, Peruzza L, Perin S, Li S, McAteer T, De Coppi P, Andrews PW, Tsakiridis A & McCann CJ (2025) Functional and molecular rescue of aganglionic colon by human enteric nervous system progenitor transplantation in Hirschsprung disease, Cold Spring Harbor Laboratory.
• Jevans B, Cooper F, Fatieieva Y, Gogolou A, Kang Y-N, Restuadi R, Berghe PV, Adameyko I, Thapar N, Andrews PW , Coppi PD et al (2023) Human enteric nervous system progenitor transplantation restores functional responses in Hirschsprung Disease patient-derived tissue, Cold Spring Harbor Laboratory.
• Cooper F, Souilhol C, Haston S, Gray S, Boswell K, Gogolou A, Frith T, Stavish D, James BM, Bose D , Dale JK et al (2023) Notch signalling influences cell fate decisions and HOX gene induction in axial progenitors, Cold Spring Harbor Laboratory.
• Butler L, Adamson KI, Johnson SL, Jestice LH, Price CJ, Stavish D, Pooranachandran N, Malicki JJ, Tsakiridis A, Grierson AJ & Barbaric I (2022) HDAC6 inhibition partially alleviates mitochondrial trafficking defects and restores motor function in human motor neuron and zebrafish models of Charcot-Marie-Tooth Disease Type 2A, Cold Spring Harbor Laboratory.
• Gogolou A, Souilhol C, Granata I, Wymeersch FJ, Manipur I, Wind M, Frith TJR, Guarini M, Bertero A, Bock C , Halbritter F et al (2021) Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor, Cold Spring Harbor Laboratory.
• Wind M, Gogolou A, Manipur I, Granata I, Butler L, Andrews PW, Barbaric I, Ning K, Guarracino MR, Placzek M & Tsakiridis A (2020) Defining the signalling determinants of a posterior ventral spinal cord identity in human neuromesodermal progenitor derivatives, Cold Spring Harbor Laboratory.
• Ruske LJ, Kursawe J, Tsakiridis A, Wilson V, Fletcher AG, Blythe RA & Schumacher LJ (2020) Coupled differentiation and division of embryonic stem cells inferred from clonal snapshots, arXiv.
• Frith TJR, Gogolou A, Hackland JOS, Barbaric I, Thapar N, Burns AJ, Andrews PW, Tsakiridis A & McCann CJ () Retinoic acid accelerates the specification of enteric neural progenitors from in vitro-derived neural crest.