Professor Tanya Whitfield

Dr Tanya Whitfield

Professor of Developmental Biology
Bateson Centre and Department of Biomedical Science
University of Sheffield
Sheffield
S10 2TN
United Kingdom

Room: C21 Firth Court
Telephone: +44 (0) 114 222 2350
email: t.whitfield@sheffield.ac.uk

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General

Brief career history

  • 2016-present: Professor of Developmental Biology
  • 2013-2015: Reader in Developmental Biology
  • 2004-2012: Senior Lecturer, University of Sheffield.
  • 1997-2004: Lecturer, University of Sheffield.
  • 1994-1997: Imperial Cancer Research Fund Postdoctoral Fellow and Linacre College Junior Research Fellow, Developmental Biology Unit, University of Oxford, and Lincoln’s Inn Fields, London. Research Advisor: Dr Julian Lewis.
  • 1994: EMBO Short Term Fellow, Max Planck Institute for Developmental Biology, Tübingen, Germany. Research Advisor: Professor Christiane Nüsslein-Volhard.
  • 1992-1994: Wellcome Postdoctoral Fellow, Department of Zoology and Wellcome Trust/Cancer Research Campaign Institute, University of Cambridge. Research Advisor: Professor Chris Wylie.
  • 1989-1992: Wellcome PhD student, Department of Zoology and Wellcome Trust/Cancer Research Campaign Institute, University of Cambridge. Research Advisor: Professor Chris Wylie.
  • 1986-1989: BA Natural Sciences (Zoology), University of Cambridge.

Research interests

My group uses the zebrafish as a model organism to study the development and function of the inner ear.

Professional activities

  • Invited speaker at national and international meetings (recent presentations at: International Light-Sheet Fluorescence Microscopy Conference 2017, Singapore; UCL Ear Institute; Institut de Biologie Paris-Seine, France; University of Nottingham; University of Edinburgh; Institute for Advanced Study, Delmenhorst, Germany; Gordon Conference 'Neural Crest and Cranial Placodes', Waltham, MA, USA; Association for Research in Otolaryngology 38th MidWinter meeting (Plenary speaker), Baltimore, MD, USA).
  • Board of Reviewing Editors, eLife
  • Associate Editor (Genetics), Royal Society Open Science
  • Member, BBSRC Core Committee C

You can follow events from the Whitfield lab on our blog or on Twitter:

Full publications


Research

Development of the fish inner ear

Summary for the non-specialist

The inner ear is the organ that mediates our senses of hearing and balance. It consists of an intricate fluid-filled labyrinth housing a variety of extraordinarily sensitive sensory structures that respond to sound, movement and gravity.

For correct inner ear function it is essential that each of these components  form in exactly the right place in the embryo, as any abnormalities can lead to  deafness or balance disorders.  Indeed, congenital deafness is an important clinical condition, affecting approximately one in every thousand children at birth.

Our aim is to understand how the inner ear develops in the embryo, and the mechanisms that ensure that the different cell types in the ear arise in the correct positions so that they can function accurately.

We use embryos of a small tropical fish, the zebrafish, in our research, as this fish is a superb model for the study of vertebrate inner ear development.

Zebrafish Ear

Dynamics of inner ear formation.  The movie shows the otic vesicle (developing ear) of a zebrafish embryo on the third day after fertilisation.  Cell membranes are labelled in green, and cell nuclei in red. 

Projections of epithelium grow into the centre of the vesicle to form the semicircular canal system.  

Movie credit: S. Baxendale and N. van Hateren.  Transgenic lines from Robert Knight and the Nieto lab.


Technical Summary

Current projects in the Whitfield lab include:

Morphogenesis and patterning in the otic placode and vesicle

We are identifying pathways of gene activity that lead to correct axial patterning of the otic epithelium, together with establishment of the neurogenic, sensory and non-sensory domains in the developing ear. We have a long-standing interest in the roles that Hedgehog, Fgf and BMP signalling play in these processes.

Development of the vestibular system

The adult inner ear consists of three interconnected ducts of non-sensory epithelium—the semicircular canals—and various chambers containing sensory hair cells.  We are examining zebrafish lines that develop with morphological defects of the semicircular canal system.  We are using light-sheet microscopy to image semicircular canal formation in these lines, and automated tracking of adult fish to measure any balance deficits.

Research figure 3

Optical section through a semicircular canal duct (circular structure, top left) and ampulla (sensory chamber) in an adult zebrafish ear.  Inner ear epithelial cells are labelled in green, and actin is stained in red.  The actin-rich hair bundles of the sensory hair cells form a bright red fringe on top of the crista, a band of sensory tissue at the base of the ampulla.  Image credit: S. Baxendale and N. van Hateren.  Transgenic line from Robert Knight.

We are also interested in the formation of the otoliths, biomineralised ‘ear stones’ that sit over patches of sensory hair cells in the ear.  In the zebrafish ear, otoliths are initially tethered to the tips of kinocilia on the sensory hair cells.  Later, they adhere to the sensory patch via the otolithic membrane.  We have recently identified two large glycoproteins involved in zebrafish otolith tethering and adhesion.

Modelling human deafness and vestibular disorders

Some of our zebrafish lines form models for a variety of human diseases, including Waardenburg-Shah syndrome, Branchio-Oto-Renal syndrome and DiGeorge syndrome.

Ototoxicity

A number of drugs used to treat human infections or cancer have ototoxic side effects: they damage sensory hair cells in the ear, leaving a patient with temporary or permanent hearing loss and vestibular impairment.  We are using the lateral line of the zebrafish embryo as a screening tool to identify compounds that can protect hair cells from the ototoxic effects of drugs such as neomycin and cisplatin.

Research figure 4

Research figure 5

Funding

Group members

Teaching

Undergraduate taught modules

Level 1:

  • BMS107/109 - Introduction to Developmental Biology (Co-ordinator)
  • BMS109 Laboratory Skills in BMS

Level 3:

  • BMS339 Patients as Educators Project
  • BMS349/359 Extended Library Project (Co-ordinator)
  • BMS369 Laboratory Research Project
Opportunities

Postdoc position available: Computational Image Analysis for Microscopy

A 12-month postdoctoral post is available, funded by the BBSRC, to investigate development of the zebrafish inner ear.  The project aims to use, develop and implement software for the computational analysis of large imaging datasets generated by light-sheet microscopy.  This will involve the use and development of novel tools for automated cell segmentation, tracking and quantitative analysis.

The post holder will work within the group of Professor Alejandro Frangi (CISTIB, Faculty of Engineering, University of Sheffield), but will also spend regular time liaising with the Whitfield lab in the Bateson Centre and Department of Biomedical Science.

The successful candidate should hold a PhD or equivalent in a relevant subject, with interest and expertise in biological image analysis, microscopy, medical image computing, computer vision or machine learning.  Experience with packages such as Fiji, Arivis and Imaris would be an advantage.  The postholder will assist in the supervision of one or more postgraduate students whose projects are closely allied to the objectives of the grant.

The post is fixed-term for 12 months, with an immediate start date.

Applications can be made directly online here (job ref. UOS017267).

For further information, please contact Alex Frangi or Tanya Whitfield.

PhD positions for 2017 entry to the lab are now filled.  

We welcome enquiries about opportunities for postdoctoral or postgraduate study in the lab.  Any funding opportunities will be listed here when available, but we also welcome enquiries from postdocs who are interested in preparing fellowship applications (e.g. Marie Sklowodska-Curie Actions - Individual Fellowships).  Please contact:

Professor Tanya Whitfield 

PhD Opportunities

Selected publications

Journal articles