Prof Jeff Green
0114 222 4403
Honours and Distinctions
Microbiology, biotechnology, catabolism, environmental signalling, oxygen sensing
Director of the Crossing Biological Membranes BBSRC Network in Industrial Biotechnology and Bioenergy (http://www.cbmnetnibb.net/)
Bacteria exploit virtually every environmental niche on Earth. This success depends upon their ability to adapt to a range of stresses imposed by an ever-changing environment. Understanding how bacteria respond to environmental change by exploiting their biochemical versatility is one of the most important challenges in microbial science. Research in my laboratory is focused on how bacteria respond to stress. We are particularly interested in how bacteria (Escherichia coli; Salmonella enterica; Microbacterium tuberculosis) sense and respond to changes in oxygen availability, and to oxidative and nitrosative stresses. These studies have led to a particular interest in characterizing signal perception, DNA recognition, and interactions with RNA polymerase for several members of the CRP-FNR family of transcription factors. In addition, we are exploiting systems approaches to learn more about the dynamics of the transcriptome as bacteria transit from one condition to another. On-going programmes are focussed on the molecular mechanisms underpinning:
Figure 1: Oxygen sensing by the bacterial transcription factor FNR. Box on the left shows a model of a FNR monomer. Incorporation of a [4Fe-4S] cluster (Fe in red, S in yellow) promotes the formation of transcriptionally active FNR homodimers. Box on the right shows the reaction of the FNR [4Fe-4S] cluster with oxygen to yield the transcriptionally inactive [2Fe-2S] form of the protein.
Module Coordinator: MBB266 Molecular Bioscience 2A
Level 4 Modules
Level 3 Modules
Level 2 Modules
I welcome applications from self-funded prospective home and international PhD students; see examples of possible projects below.
You can apply for a PhD position in MBB here.
Contact me at firstname.lastname@example.org for further information.
- Ibrahim SA, Crack JC, Rolfe MD, Acuña JMB-D, Thomson AJ, Le Brun NE, Schobert M, Stapleton MR & Green J (2015) Three Pseudomonas putida FNR Family Proteins with Different Sensitivities to O 2. Journal of Biological Chemistry, 290(27), 16812-16823. View this article in WRRO
- Denby KJ, Rolfe MD, Crick E, Sanguinetti G, Poole RK & Green J (2015) Adaptation of anaerobic cultures of Escherichia coliK-12 in response to environmental trimethylamine-N-oxide. Environmental Microbiology, 17(7), 2477-2491. View this article in WRRO
- Crack JC, Green J, Thomson AJ & Brun NEL (2014) Iron–Sulfur Clusters as Biological Sensors: The Chemistry of Reactions with Molecular Oxygen and Nitric Oxide. Accounts of Chemical Research, 47(10), 3196-3205.
- Bai H, Rolfe MD, Jia W, Coakley S, Poole RK, Green J & Holcombe M (2014) Agent-based modeling of oxygen-responsive transcription factors in Escherichia coli.. PLoS Comput Biol, 10(4), e1003595. View this article in WRRO
- Crack JC, Stapleton MR, Green J, Thomson AJ & Le Brun NE (2013) Mechanism of [4Fe-4S](Cys)
4cluster nitrosylation is conserved among NO-responsive regulators. Journal of Biological Chemistry, 288(16), 11492-11502.
- Smith LJ, Stapleton MR, Buxton RS & Green J (2012) Structure-function relationships of the Mycobacterium tuberculosis transcription factor WhiB1.. PLoS One, 7(7), e40407. View this article in WRRO
- Zhang B, Crack JC, Subramanian S, Green J, Thomson AJ, Le Brun NE & Johnson MK (2012) Reversible cycling between cysteine persulfide-ligated [2Fe-2S] and cysteine-ligated [4Fe-4S] clusters in the FNR regulatory protein. Proceedings of the National Academy of Sciences of the United States of America, 109(39), 15734-15739.
- Rolfe MD, Ter Beek A, Graham AI, Trotter EW, Asif HMS, Sanguinetti G, de Mattos JT, Poole RK & Green J (2011) Transcript profiling and inference of Escherichia coli K-12 ArcA activity across the range of physiologically relevant oxygen concentrations.. J Biol Chem, 286(12), 10147-10154.
- Smith LJ, Stapleton MR, Fullstone GJM, Crack JC, Thomson AJ, Le Brun NE, Hunt DM, Harvey E, Adinolfi S, Buxton RS & Green J (2010) Mycobacterium tuberculosis WhiB1 is an essential DNA-binding protein with a nitric oxide-sensitive iron-sulfur cluster.. Biochem J, 432(3), 417-427.
- Stapleton M, Haq I, Hunt DM, Arnvig KB, Artymiuk PJ, Buxton RS & Green J (2010) Mycobacterium tuberculosis cAMP receptor protein (Rv3676) differs from the Escherichia coli paradigm in its cAMP binding and DNA binding properties and transcription activation properties.. J Biol Chem, 285(10), 7016-7027.
- Jervis AJ, Crack JC, White G, Artymiuk PJ, Cheesman MR, Thomson AJ, Le Brun NE & Green J (2009) The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversion.. Proc Natl Acad Sci U S A, 106(12), 4659-4664.
- Ryan RM, Green J, Williams PJ, Tazzyman S, Hunt S, Harmey JH, Kehoe SC & Lewis CE (2009) Bacterial delivery of a novel cytolysin to hypoxic areas of solid tumors.. Gene Ther, 16(3), 329-339.