Dr Anton Nikolaev
Lecturer in Neuroscience
Room: D232 Alfred Denny building
Brief career history
Our research is focused on investigating the neuronal circuits responsible for information processing in the visual system. The vertebrate visual system is able to recognize a remarkable number of objects of different appearances but the mechanisms and neural circuits underlying this ability are not known. To tackle this problem we use in vivo imaging of neuronal activity in zebrafish and follow the processing of visual information in different brain areas.
Neuronal circuits involved in processing of visial information in zebrafish
1. To understand the organisation of neuronal circuits performing processing of visual information in zebrafish. Using a combination of behavioural and imaging techniques we study how the zebrafish visual system processes visual information. Our main goal is to understand how information about object identity is encoded in the activity of visual neurons. The range of questions we ask includes: what features are extracted by the early visual system in order to make object recognition efficient? How do these features converge to form receptive fields of object recognising neurons? What is the role of adaptation in this process?
To answer these questions we image neuronal activity in zebrafish larvae. We are using zebrafish lines expressing calcium activity indicators in all or subset of visual neurons. These indicators change their brightness when neuron is active. The advantage of this method is that it is non-invasive and allows for the simultaneous study of a large population of neurons - something that is currently unfeasible using other techniques.
2. To understand how memory is encoded in changes in synaptic strength. We are develop GFP based reporters of long-term potentiation and long-term depression. These reporters will be used in vivo to understand how simple forms of associative and non-associative memory are implemented in changes in synaptic strength. To answer these questions we are developing behavioural paradigms that will allow us to combine evaluation of memory formation with in vivo imaging of synaptic strength.
2015: Postgraduate Certificate in Learning and Teaching from the University of Sheffield (Fellow of The Higher Education Academy, FHEA)
Undergraduate and postgraduate taught modules
Interaction of visual and olfactory systems in the zebrafish brain
Supervisors: Dr Anton Nikolaev and Dr Suresh Jesuthasan
Funding status: A fully funded PhD studentship is available for a highly motivated student to work on interactions between visual and olfactory systems in zebrafish. This is a collaborative project between Dr. Anton Nikolaev’s lab in the University of Sheffield and Dr. Suresh Jesuthasan’s lab in the Institute of Molecular and Cell Biology in Singapore.
The main aim of the proposed PhD project is to address how processing of visual information is affected by the olfactory input using zebrafish larvae as a model organism. Using a combination of behavioural techniques, 2-photon imaging of neuronal activity and advanced data analysis, you will study how the tuning properties of visual neurons in zebrafish are affected by the olfactory neurons responsible for detection of various behaviourally relevant odours.
The specific objectives of the project are:
Eligibility and Entry Requirements
The project is for students with biological, engineering or physical sciences background with 1st or 2:1 Bachelor’s degree from UK University or an equivalent. The position is for UK/EU citizens only.
You must also be willing to travel: in the first half of the study you will work under supervision of Dr. Nikolaev in Sheffield and then move to Singapore to work under supervision of Dr. Jesuthasan. All travel expenses will be covered.
For informal enquiries about the project or application process, please feel free to contact Dr Anton Nikolaev
or Suresh Jesuthasan ( email@example.com )
For further information about this project, and how to apply, see our PhD Opportunities page:
- Razlivanov I, Liew T, Moore EW, Al-Kathiri A, Bartram T, Kuvshinov D & Nikolaev A (2018) Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured cells. BioTechniques, 65(1), 37-39.
- Bergmann K, Meza Santoscoy P, Lygdas K, Nikolaeva Y, MacDonald R, Cunliffe V & Nikolaev AV (2018) Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. Journal of Developmental Biology, 6(1). View this article in WRRO
- Baden T, Nikolaev A, Esposti F, Dreosti E, Odermatt B & Lagnado L (2014) A Synaptic Mechanism for Temporal Filtering of Visual Signals. PLoS Biology, 12(10). View this article in WRRO
- Nikolaev A, Leung KM, Odermatt B & Lagnado L (2013) Synaptic mechanisms of adaptation and sensitization in the retina. Nature Neuroscience, 16(7), 934-941.
- Zheng L, Nikolaev A, Wardill T, O'Kane C, de Polavieja GG & Juusola M (2009) Network Adaptation Improves Temporal Representation of Naturalistic Stimuli in Drosophila Eye: I Dynamics. PLoS One, 4(1). View this article in WRRO
- Odermatt B, Nikolaev A & Lagnado L () Encoding of Luminance and Contrast by Linear and Nonlinear Synapses in the Retina. Neuron, 73(4), 758-773.