Dr Nicolas Viphakone
T: 0114 222 2749
I am interested in gene expression in humans, RNA biology in general, and their dysfunctions associated with human pathologies.
The major steps allowing expression of a eukaryotic gene encoding a protein consist of :
Gene -> nuclear pre-mRNA synthesis and maturation into messenger RNA (mRNA) -> export to the cytoplasm -> translation into proteins.
Cellular localisation of mRNAs in a human cell. The pre-mRNA is synthesised in the nucleus from the DNA template (blue). After various biochemical modifications, the mature polyadenylated mRNA (red) is exported through the nuclear membrane to the cytoplasm to be translated into proteins. Ultimately, the mRNA, which constitutes a copy of the genetic information encoded in the nuclear DNA molecule, will be degraded in the cytoplasm after being translated.
My current research interests in the field of gene expression are:
- pre-mRNA processing and its regulations.
- mRNP formation and nucleocytoplasmic mRNA transport in human cells.
Heath CG, Viphakone N, Wilson SA. The role of TREX in gene expression and disease. Biochem J. 2016 Oct 1;473(19):2911-35. doi: 10.1042/BCJ20160010. Review. PubMed ID: 27679854.
Viphakone N, Cumberbatch MG, Livingstone MJ, Heath PR, Dickman MJ, Catto JW, Wilson SA. Luzp4 defines a new mRNA export pathway in cancer cells. Nucleic Acids Research 2015 Feb 27;43(4):2353-66. doi: 10.1093/nar/gkv070. PubMed ID: 25662211
Chang CT, Hautbergue GM, Walsh MJ, Viphakone N, van Dijk TB, Philipsen S, Wilson SA. Chtop is a component of the dynamic TREX mRNA export complex. EMBO J. 2013 Feb 6;32(3):473-86. doi: 10.1038/emboj.2012.342. PubMed ID: 23299939
Viphakone N, Hautbergue GM, Walsh M, Chang CT, Holland A, Folco EG, Reed R, Wilson SA. TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export. Nature Communications. 2012;3:1006. doi: 10.1038/ncomms2005. PubMed ID: 22893130
Haddad R, Maurice F, Viphakone N, Voisinet-Hakil F, Fribourg S, Minvielle-Sébastia L. An essential role for Clp1 in assembly of polyadenylation complex CF IA and Pol II transcription termination. Nucleic Acids Research 2012
Feb;40(3):1226-39. doi: 10.1093/nar/gkr800. PubMed ID: 21993300
Viphakone N, Voisinet-Hakil F, Minvielle-Sebastia L. Molecular dissection of mRNA poly(A) tail length control in yeast. Nucleic Acids Research 2008 Apr;36(7):2418-33. doi: 10.1093/nar/gkn080. PubMed ID: 18304944
Dheur S, Nykamp KR, Viphakone N, Swanson MS, Minvielle-Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. Journal of Biological Chemistry. 2005 Jul 1;280(26):24532-8. PubMed ID: 15894541.